EDITOR IN CHIEF QUALIFICATION 

1993 B.Sc. Veterinary Medicine 

Faculty of Veterinary Medicine, Cairo University, Egypt. 

1999 M.Sc. Bacteriology, Mycology & Immunology 

Faculty of Veterinary Medicine, Cairo University, Egypt. 

Thesis Title: Effect of Bacillus thuringiensis on Eggs of Some Gastrointestinal Helminths in Animals. 

2003 Ph.VMD. Bacteriology, Mycology & Immunology 

Faculty of Veterinary Medicine, Cairo University, Egypt. 

Thesis Title: Cytocidal Activity of Bacillus thuringiensis Crystal Proteins. 

Distinction: 

M.Sc. and Ph.VMD. degrees are accredited from Josef Silny International Educational Consultant, Miami, USA. 

WORK EXPERIENCE 

1995-1999 Research Assistant 

Microbiology and Immunology Department, National Research Center, Cairo, Egypt. 

Duration: 04 Years 

1999-2003 Assistant Researcher 

Microbiology and Immunology Department, National Research Center, Cairo, Egypt. 

Duration: 04 Years 

2003-Jan 2009 Researcher 

Microbiology and Immunology Department, National Research Center, Cairo, Egypt. 

Duration: 06 Years 

Feb 2009-Jun 2018 Assistant Professor 

Microbiology and Immunology, College of Pharmacy, Jazan University, Jazan, Saudi Arabia. 

Duration: 09 Years 

Aug 2018-Jan 2020 Researcher 

Microbiology and Immunology Department, National Research Center, Cairo, Egypt. 

Duration: 1.5 Years 

Feb 2020-To date. Associated Professor 

Microbiology and Immunology Department, National Research Center, Cairo, Egypt. 

Duration: 04 Years 

PATENTS 

Patent No. 24253 

“Preparation of Parasporal Inclusion Proteins from Bacillus thuringiensis for us as Anticancer Agent” 

Authority: Egyptian patent office, Academy of Scientific Research and Technology, Cairo, Egypt. 

Patent No. 4668 

‘Antimicrobial Peptide Isolated from Xenorhabdus Indica” 

Authority: Saudi Authority for Intellectual Property, Saudi Patent Office, Saudi Arabia. 

Gen Bank Accession Number: KU317677 

Patent No. 4680 

“Pharmaceutical Composition for Topical Treatment of the Eye Infection” 

Authority: Saudi Authority for Intellectual Property, Saudi Patent Office, Saudi Arabia. 

JOURNAL REVIEWER 

Working as Reviewer of the following Journals. 

1. Letters in Applied Microbiology. 

2. Journal of Applied Microbiology. 

CONFERENCES 

1. “Nutrition and Cancer” organized Cancer Biology Dept., National Cancer Institute, Cairo University. From 26 – 29th December 2003. 

2. “Educational course on Genitourinary Cancer” organized by European Society of Medical Oncology in collaboration with National Cancer Institute, Cairo University, from 26 – 29th Nov. 2005. 

WORKSHOPS 

1. “Natural Treatment of Drinking Water” In Suez Canal University – Ismailia in coordination with DAAD. 

2. “From Gene to Function” In Marian University, Fond du Lac, WI – USA under supervision of Dr. Susan Bornstein from 25th July to 25th August 2008. 

3. Collaborative research conducted with Dr. Steven Forst, Professor of biological Science from July 25 – August 27, 2008 in Department of Biological science, University of Wisconsin-Milwaukee- USA. 

EXPERTISE 

• • Immunological studies and research included immunoassays, immunotherapy and immunodiagnostic assays. 
• • Bacterial endotoxin and exotoxins extraction, purification and testing. 
• • Tissue culture and tumor biology. 
• • Applied microbiology included bacteriological culture and bacterial identification (bacterial morphological and biochemical identification) 
• • Antimicrobial agents and antibiotics purification, testing and detection of antibiotic resistance gene. 
• • Formulation and testing of antimicrobial agents. 
• • Long term and short-term viability assays. 
• • Antibiotics stability tests. 
• • Antibiotic resistance assays. 
• • ELISA assays. 
• • PCR identification. 
• • Immunofluorescence assays. 
• • Tissue culture passage. 
• • Bacterial fermentation. 

TEACHING EXPERIENCE 

Training Course in ‘Tissue Culture and Evaluation of Anticancer Agents.’ 

Department of Natural Science and Life, Faculty of Science, University of Mintouri, Constantine, Algeria. 

(2005-2008) 

Training Course in ‘Biological Application of Entomopathogenic Nematodes and Its Symbiotic Bacteria.’ 

Post Graduate Students from Brazil. 

(March-April 2008) 

Training Course in ‘Identification of Phenotyping, Biotyping, and Serotyping.’ and ‘Microbial Products Toxins and Proteins.’ 

Ain Shams University, Cairo Egypt. / Cairo University, Cairo Egypt. / Helwan University, Helwan, Egypt. /Tanta University, Tanta, Egypt. 

(Summer Courses) 

Training Course in ’Basic & Diagnostic Immunology, Diagnostic Bacteriology, Pharmaceutical Microbiology and Molecular Biology.’ 

Jazan University, Jazan, Saudi Arabia. 

(2009-2018) 

RESEARCH PUBLICATIONS 

1. “Studies on the parasporal inclusion proteins of Bacillus thuringiensis serovar dakota (H15): I – Production, Isolation, Characterization and Activation”. Journal of Union of Arab Biologist Cairo 19(A): Zoology, 153-175. 

2. “Studies on the parasporal inclusion proteins of Bacillus thuringiensis serovar dakota (H15): II- In vitro studies on mammalian cancer cell lines”. Journal of Union of Arab Biologist Cairo 19(A): Zoology, 39-54. 

3. Studies on the parasporal inclusion proteins of Bacillus thuringiensis serovar dakota (H15): III- In vivo studies on mammalian cancer cell lines.” European Society of Medical Oncology on Collaboration with National Cancer Institute – Cairo Univ. “Educational Course on Genitourinary Cancer” (Abstract). 

4. “Effect of grape and grapefruit juice extracts on cancer cells in vitro” Egyptian Journal of Applied Science, 21 (10A): 83 – 97. 

5. “Biological treatment of biological contaminants in drinking water” in “Natural Treatment of Drinking Water” In Suez Canal University – Ismailia in coordination with DAAD in 12- 12- 2007. 

6. “XENOPROTEC: ANTIMICROBIAL AGENT DERIVED FROM Xenorhabdus indica” American – Eurasian Journal of Agriculture and Environmental Science. 3 (4): 568-576. 

7. “In Vitro activity of Bacillus thuringiensis (H14) 43 kDa crystal protein against Leishmania major” American – Eurasian Journal of Agriculture and Environmental Science. 3 (4): 583-589. 

8. “Selective anti-malignancy activity of Paracoccus alcaliphilus proteins against mammalian cell lines in vitro.” American – Eurasian Journal of Agriculture and Environmental Science. 3 (6): 842-849. 

9. “Ultrastructural changes in hemocyte cells of Hard tick (Hyalomma dromedarii: Ixodidae): A model of Bacillus thuringiensis var. thuringiensis H14 δ-endotoxin mode of action.” American – Eurasian Journal of Agriculture and Environmental Science. 3 (6) 

10. “Antimalignancy Activity of Bacillus thuringiensis Serovar dakota (H15) in vivo” World Journal of Medical Sciences. 6 (1): 06-16. 

11. “Agarose slid EIA for diagnosis of HCV-Ab in rural areas” (Abstract) American Association of Immunologist 98th Annual meeting in San Francisco from 13th 18th May 2011. 

12. Agarose slid EIA for diagnosis of HCV-Ab in. rural areas” (full text) Journal of Life Science, 10 (1) 1879-1884. 

13. Natural Associations between symbionts Photorhabdus spp. and Xenorhabdus spp. and bacteria related to Ochrobactrum anthropi, Bacillus pumilus and Enterobacter cloacae. Journal of Life Science, 9 (4) 4783 – 4790. 

14. “Assessment of immune response against HIV-1 P24 antigen using Bacillus thuringiensis serovar dakota (H15) activated deltaendotoxin as an adjuvant” (Abstract) American Association of Immunologist 100th Annual meeting in Hawaii from 3rd – 7th May 2013. 

15. “Xenohagxin: New Proteinaceous Antibiotic Derived from Xenorhabdus indica. (Abstract) 54th ICAAC 2014 Washington DC. Xenohagxin had been registered in Saudi Patent Office on 10th June 2014 with Registration Number: 34470. 

16. Xenoptec: New Ocular Formula for Treatment of External Ocular Bacterial Infection. (Abstract) ICAAC 2015 San Diego CA. 18 – 21, Sept. 2015. 

17. “Molecular and Phenotypic Characterization of Two Bacteria Photorhabdus luminescens subsp. akhurstii HRM1 and HS1 Isolated from Two Entomopathogenic Nematodes Heterorhabditis indica RM1 and Heterorhabditis sp. S1″ Pakistan J. Zool., vol. 48(1), pp. 51- 

18. “Nanoparticle System for Anticancer Drug Delivery: Targeting to Overcome Multidrug Resistance” in “Multifunctional Systems for Combined Deliver Biosensing and Diagnostics”. (Grumezescu A.M. Eds.), pp. 159 – 169. Elsevier Inc. ISBN: 978-0-323-52725-5. 

19. “Preparation and physical characterization of Cisplatin Chitosan nanoparticles by Zeta nanosizer (prime step for formulation and development)”. International Journal of Pharmaceutical Sciences and Research (IJPSR), Vol. 8(10): 4245-4249. 

20. Assessment of Immunomodulatory Effects of Black Cumin Seed (Nigella Sativa) Extract on Macrophage Activity in vitro. International Journal of Veterinary Science. Vol. 8 (4): 385-389. 

21. Detection of virulence and multidrug resistance operons in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat. Vet World. 12(10): 1524–1528. 

22. Advanced Identification and Characterization of Listeria Species in Egyptian Local Soft Cheese. South Asian Journal of Research in Microbiology. 8 (2) :11-18. 

23. Molecular Divergence of Staphylococcus aureus Isolated from Dogs and Cats. Jordan Journal of Biological Sciences Volume 13 (2) 

24. Prevalence, Antibiogram Pattern and Virulence Genes Profile of Bacillus cereus Isolated from Buffalo Milk. International Journal of Veterinary Science 10(3): 234-239. 

RESEARCH PROJECTS 

1. “Biochemical and molecular studies on the camel tick Hyalomma dromedarii“, 1998 – 2001 Agreement No. 1-1- 2-1-6 between Molecular Biology Dept. and National Research Center. 

2. Biochemical control of the camel tick Hyalomma dromedarii“, 2001 – 2004 Agreement No. 10- 43 – 5 between Molecular Biology Dept. and National Research Center. 

3. “Production of alkaline phosphatase from the liver of some mammals for the use in medical applications” 2004 – 2007 Agreement No. 7- 11-0-1-0-1 between Molecular Biology Dept. and National Research Center. 

4. “Production of superoxide dismutase enzyme from the liver of some mammals on pilot scale for use in medical applications”, 2007 – 2010 between Molecular Biology Dept. and National Research Center. 

5. “Studies on Xenorhabdus and Photorhabdus antibiotic activities against pathogens of medical and veterinary importance”, 2007 – 2009. Agreement No. 1-1-2-1-6 between Department of Biological Science University of Wisconsin Milwaukee, WI 53201, USA and National Research Center through the US-Egyptian Joint. 

6. “Production and formulation of novel antibiotic for treatment of ocular microbial infection from Xenorhabdus bacteria” for 12 months starting from 12-12-2014. Agreement No. 3465 with the Research Deanship- Jazan University.

7. “Targeting Hepatocellular carcinoma by Sorafenib loaded chitosan nanoparticles surface linked Cetuximab” for 12 months starting from 12-12-2014. Agreement No. 2991 with the Research Deanship- Jazan University, Saudi Arabia.